We have developed a patented cutting-edge single-objective Selective Plane Illumination Microscope (soSPIM) allowing 3D imaging of biological samples at high- and super-resolution and at various temporal scales. It relies on microfabricated coverslips embedding arrays of microwells flanked with micromirrors that enable the creation of a light-sheet perpendicular to the objective’s optical axis. We demonstrated its capability to image biological samples of various dimensions (from single cell, cell doublet, cell aggregates up to drosophila embryo) with very good spatial and temporal resolutions, down to the single-molecule level.

We initiated several academic collaborations through Material Transfer Agreement and have the project to create a startup company, REFLECT, to industrialize and distribute the soSPIM technology. (Galland et al., Nature Methods 2015, Singh et al., Biophys. J., 2017).

We are extending the Single Molecule Localization Microscopy (SMLM) imaging capabilities of the soSPIM Technology in order to investigate protein organization and dynamics in extended volumes, with single-molecule resolution. 3D multicolor aberration-free SMLM at long distance range from the coverslip is achieved in combination with Adaptive Optics (AO) device. Positioned in the emission path of the system, AO is used i) to optimize the signal by correcting the optical aberrations, and ii) to add a pure well-controlled astigmatism for 3D localization. Aberration corrections in depth and automatic volumetric 3D SMLM acquisition and registration is achieved recording the single molecule signal to sparse nano-diamonds embedded into the microchip polymers.