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Synaptic Plasticity and Super-Resolution Microscopy

Team Leader : Valentin Nägerl

Bienvenue! Welcome! Willkommen! Yōkoso! Powitanie! Benvenuti! Dobro pozhalovat!

I am a full Professor of Neuroscience and Bioimaging and lead the research group 'Synaptic Plasticity and Super-Resolution Microcopy' at the Interdisciplinary Institute for NeuroScience. I also help run the Graduate School for Light Science and Technologies (EUR Light S&T) at the University of Bordeaux.

After undergrad studies in physics and medicine at the University of Göttingen, I got my PhD in neuroscience from UCLA with Istvan Mody and did a postdoc at the Max Planck Institute of Neurobiology with Tobias Bonhoeffer, spending research visits at the Max Planck Institute for Biophysical Chemistry with the Nobel laureate-to-be Stefan Hell. In 2009, I obtained the habilitation for neuroscience from the Technical University of Munich with Arthur Konnerth, before setting up my own laboratory in Bordeaux. In 2017, I became a senior member of the 'Institut Universitaire de France' (IUF), and our work has been recognized by an 'Equipe FRM' award (2016), a Research Prize from the French Academy of Sciences (2018) and the Maxime Dahan Prize (2023) from the Curie Institute. In addition, I have received two highly competitive HFSP program awards (in 2010 and 2020). In 2021, I was promoted to the rank of 'Professeur classe exceptionelle' (PRCE1). 

My team is working at the interface of optical method development and neuroscience research, animated by the siren call and possibilities of new technology, academic freedom and the joy of tackling challenging projects together with wonderful collaborators here in Bordeaux and across the globe. The team is relatively small, but we have a good fighting spirit and great working conditions at Bordeaux NeurocampusDriven by curiosity and instinct, we seek to decipher the enigmatic wonders of the brain and to help get the next generation of neuroscientists excited and ready for this mission.

Over the next few years, our research will be funded by the Agence Nationale de la Recherche (ANR), Human Frontier Science Program (HFSP), European Research Council (ERC-Synergy) and Wellcome Trust (Discovery Award), giving me a fantastic chance to realize some of my wilder scientific dreams.

General objective

The biology of synapses is an extremely productive and interdisciplinary scientific endeavor, harboring central questions of cell biology and neuroscience. Synapses are physical sites of intercellular contact that transmit and transform information in a very rapid and flexible way, playing a pivotal role for learning and memory formation as well as neurological diseases of the mammalian brain. Since synapses are tiny and densely packed in light-scattering brain tissue, understanding their dynamic behavior in mechanistic terms under physiological conditions is a serious experimental challenge.

Fortunately, recent technological innovations, particularly in labeling and live-cell imaging techniques, are helping to break new ground. The advent of fluorescence microscopy beyond the diffraction limit has opened up huge experimental opportunities to directly image and resolve key physiological signaling events inside single synapses in intact brain tissue, a possibility which was considered a pipe dream until recently. My group is invested in harnessing these exciting technological developments to study synapses in their natural habitat and under realistic conditions, aiming to better understand higher brain function and disorders in terms of the underlying synaptic mechanisms.

To this end, we are developing and applying powerful super-resolution microscopy approaches (STED microscopy), giving us a much more complete and refined view of the dynamic behavior and plasticity of neuronal synapses and their interactions with glia cells inside living brain slices and in the intact mouse brain in vivo. Recently, we invented super-resolution shadow imaging (SUSHI), a new technique to visualize the extracellular space of the brain (ECS), which is an important, however understudied, brain compartment for neural signaling and brain homeostasis. These core approaches are complemented by a combination of 2-photon imaging & photoactivation, patch-clamp electrophysiology and mathematical modeling aided by tools from molecular genetics and computational image analysis.

Check out our papers on PubMed, BioRXivOrcid, Google scholar.

Research Projects

Contribution of IP3 receptors and endoplasmic reticulum to calcium signalling in astrocytes


Role of astrocyte aquaporin-4 in brain structure remodeling after traumatic brain injury: a combined MRI and STED microscopy study


Multi-scale investigation of synaptic dysfunction after stroke


Nanoscale neuro-anatomical determinants of synaptic strength, plasticity and integration


Presynaptic plasticity in hippocampal circuits


Structure and functions of the brain extracellular space


The extracellular space of the brain: a multi-modal analysis from nano-structure to in vivo function



Award of ERC Synergy Grant





Together with my Bordeaux colleagues (E. Bezard, L. Cognet and L. Groc), I am a proud recipient of an ERC Synergy grant from the EU, which supports high-risk/high gain frontier research in Europe. This is a tremendous recognition of our work, which will give us resources and wings to conduct some cool and ground-breaking research in the years to come! I am extremely grateful to all the members of my team and collaborators who have contributed to this success over the years and made it possible.

Selected Publications

  • Grassi D, Idziak A, Lee A, Calaresu I, Sibarita JB, Cognet L, Nägerl UV, Groc L
  • Nanoscale and functional heterogeneity of the hippocampal extracellular space Cell Reports (2023)

  • Bancelin S, Mercier L, Roos J, Belkadi M, Pfeiffer T, Kim SK, Nägerl UV
  • Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy Neurophotonics (2023)

  • Dembitskaya Y, Boyce AKJ, Idziak A, Pourkhalili Langeroudi A, Arizono M, Le Bourdellès G, Girard J, Ducros M, Sato-Fitoussi M, Ochoa de Amezaga A, Oizel K, Bancelin S, Mercier L, Pfeiffer T, Thompson RJ, Kim SK, Bikfalvi A, Nägerl UV
  • Shadow imaging for panoptical visualization of living brain tissue Nature Communications (2023)

  • Valiente-Gabioud AA, Garteizgogeascoa Suñer I, Idziak A, Fabritius A, Angibaud J, Basquin J, Nägerl UV, Singh SP, Griesbeck O
  • Fluorescent sensors for imaging interstitial calcium Nature Communications (2023)

  • Idziak A, Inavalli VVGK, Bancelin S, Arizono M, Nägerl UV
  • The impact of chemical fixation on the microanatomy of mouse brain tissue eNeuro (2023)

  • Misa Arizono, U. Valentin Nägerl
  • Deciphering the functional nano-anatomy of the tripartite synapse using stimulated emission depletion microscopy Glia (2022)

  • Nägerl et al.
  • Neurophotonic tools for microscopic measurements and manipulation: status report Neurophotonics (2022)

  • Misa Arizono, Agata Idziak, Federica Quici, U. Valentin Nägerl
  • Getting sharper: the brain under the spotlight of super-resolution microscopy Trends in Cell Biology (2022)

  • Nägerl et al.
  • The glymphatic system: Current understanding and modeling iSience (2022)

  • Audrey Denizot, Misa Arizono, U. Valentin Nägerl, Hugues Berry, Erik De Schutter
  • Control of calcium signals by astrocyte nanoscale morphology at tripartite synapses Glia (2022)

  • Martin Fuhrmann, Nala Gockel, Misa Arizono, Yulia Dembitskaya, U. Valentin Nägerl, Francesca Pennacchietti, Martina Damenti, Ilaria Testa, Katrin I. Willig
  • Super-Resolution Microscopy Opens New Doors to Life at the Nanoscale Journal of Neuroscience (2022)

  • Cassereau J, Courdavault V, Nägerl UV
  • Turning up the green light Trends in Cell Biology (2021)

  • Arizono M, Bancelin S, Bethge P, Chéreau R, Idziak A, Inavalli VVGK, Pfeiffer T, Tønnesen J, Nägerl UV
  • Nanoscale imaging of the functional anatomy of the brain Neuroforum (2021)
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  • Arizono M, Inavalli VVGK, Bancelin S, Fernández-Monreal M, Nägerl UV
  • Super-resolution shadow imaging reveals local remodelling of astrocytic microstructures and brain extracellular space after osmotic challenge Glia (2021)

  • Arizono M, Idziak A, Nägerl UV
  • It takes three to tango [Article in French] Médecine / Sciences (2021)

  • Bancelin S, Mercier L, Murana E, Nägerl UV
  • Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue Neurophotonics (2021)

  • Stéphane Bancelin, Luc Mercier, Emanuele Murana, U Valentin Nägerl
  • Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue Neurophotonics (2021)

  • Imig C, López-Murcia FJ, Maus L, García-Plaza IH, Mortensen LS, Schwark M, Schwarze V, Angibaud J, Nägerl UV, Taschenberger H, Brose N, Cooper BH.
  • Ultrastructural imaging of activity-dependent synaptic membrane-trafficking events in cultured brain slices Neuron (2020)

  • Henneberger C, Bard L, Panatier A, Reynolds JP, Kopach O, Medvedev NI, Minge D, Herde MK, Anders S, Kraev I, Heller JP, Rama S, Zheng K, Jensen TP, Sanchez-Romero I, Jackson CJ, Janovjak H, Ottersen OP, Nagelhus EA, Oliet SHR, Stewart MG, Nägerl UV, Rusakov DA.
  • LTP induction boosts glutamate spillover by driving withdrawal of perisynaptic astroglia Neuron (2020)

  • Arizono M, Inavalli VVGK, Panatier A, Pfeiffer T, Angibaud J, Levet F, Ter Veer MJT, Stobart J, Bellocchio L, Mikoshiba K, Marsicano G, Weber B, Oliet SHR, Nägerl UV
  • Structural basis of astrocytic calcium signals at tripartite synapses Nature Communications (2020)

  • Stolp B, Thelen F, Ficht X, Altenburger LM, Ruef N, Inavalli VVGK, Germann P, Page N, Moalli F, Raimondi A, Keyser KA, Seyed Jafari SM, Barone F, Dettmer MS, Merkler D, Iannacone M, Sharpe J, Schlapbach C, Fackler OT, Nägerl UV, Stein JV
  • Salivary gland macrophages and tissue-resident CD8+ T cells cooperate for homeostatic organ surveillance Science Immunology (2020)

  • Levet F, Tønnesen J, Nägerl UV, Sibarita JB
  • SpineJ: A software tool for quantitative analysis of nanoscale spine morphology Methods (2020)

  • Arizono M, Bancelin S, Nägerl UV
  • Thinking outside the cell: Super-resolution shadow imaging (SUSHI) of the extracellular space of the brain [Article in French] Médecine / Sciences (2020)

  • Angibaud J, Mascalchi P, Poujol C, Nägerl UV
  • A simple tissue clearing method for increasing the depth penetration of STED microscopy of fixed brain slices Journal of Physics D: Applied Physics (2020)

  • Inavalli VVGK , Lenz MO, Butler C, Angibaud J, Compans B, Levet F, Tonnesen J, Rossier O, Giannone G, Thoumine O, Hosy E, Choquet E, Sibarita JB, Nägerl UV
  • A super-resolution platform for correlative live single-molecule imaging and STED microscopy Nature Methods (2019)

  • Denizot A, Arizono M, Nägerl UV, Soula H, Berry H
  • Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity PLoS Computational Biology (2019)

  • Arizono M, Nägerl UV
  • ライブ超解像イメージングによる三者間シナプスのアストロサイト信号の解明 (Investigating astrocytic calcium signals at tripartite synapses using super-resolution microscopy) [Article in Japanese] Journal of Experimental Medicine (Jikken Igaku) (2019)
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  • Nägerl UV
  • Schattenriss des Gehirns [Article in German] Gehirn und Geist / Spektrum der Wissenschaft (2019)
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  • Hrabetova S, Cognet L, Rusakov DA, Nägerl UV
  • Unveiling the extracellular space of the brain: from super-resolved microstructure to in vivo function Journal of Neuroscience (2018)

  • Pfeiffer T, Poll S, Bancelin S, Angibaud J, Inavalli VK, Keppler K, Mittag M, Fuhrmann M, Nägerl UV
  • Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo eLife (2018)

  • Tønnesen J, Inavalli VVGK, Nägerl UV
  • Super-resolution imaging of the extracellular space in living brain tissue Cell (2018)

  • Chéreau R, Saraceno GE, Angibaud J, Cattaert D, Nägerl UV
  • Super-resolution imaging reveals activity-dependent plasticity of axon morphology linked to changes in action potential conduction velocity PNAS (2017)

  • Panatier A, Arizono M, Nägerl UV
  • Dissecting tripartite synapses with STED microscopy Philos Trans R Soc Lond B Biol Sci (2014)

  • Tønnesen J, Katona G, Rózsa B, Nägerl UV
  • Spine neck plasticity regulates compartmentalization of synapses Nature Neuroscience (2014)

  • Bethge P, Chéreau R, Avignone E, Marsicano G, Nägerl UV
  • Two-photon excitation STED microscopy in two colors in acute brain slices Biophysical Journal (2013)


    « Researcher »

    NÄGERL Valentin Researcher +33533514707

    « Technical Staff »

    BRUGIERE Thibault Technical staff +33533514700
    GAIKWAD Pranay Technical staff +33533514700
    GIRARD Jordan Technical staff +33533514748

    « Postdoc »

    DEMBITSKAYA Yulia Postdoc +33533514700
    JOSE Ashly Postdoc +33533514700
    OKUDA Kosuke Postdoc +33533514700

    « PhD student »

    GEORGET Emmanuelle PhD student +33533514700

    « Alumni & Guests »

    Visitors and guests (affiliation, position and dates)

    • Dr. Motohiro Nozumi (Nigata University, assistant professor, 06/2020 - 07/2021)
    • Dr. Yulia Dembitskaya (Collège de France, postdoc, 05 - 06/2021) 
    • Noemie Zerrouki (Université de Lille, undergrad internship student, 05/2021)
    • Prof. Sun Kim
    • Prof. Peter C. Kind
    • Dr. Angeles Almeida
    • Dr. Kohei Otomo
    • Dr. Julia Pinho
    • Dr. Nikolai Otmakhov

    Former team members (position at the time, dates, current position and contact)

    • Fabien Nadrigny (postdoc, June 2009 - 06/2011, high-school teacher)
    • Elena Avignone (assistant professor, 09/2009 - 09/2014, assistant professor,
    • Marion Jany (technician, 09/2011 - 09/2013)
    • Julie Angibaud (technician/lab engineer, 09/2010 - 12/2019, lab engineer/manager,
    • Jan Tonnesen (postdoc, 09/2010 - 09/2016, group leader at Basque Center for Neuroscience,
    • Ronan Chereau (technician then PhD student, 09/2009 - 09/2015, postdoc at University of Geneva,
    • Phillip Bethge (PhD student, 09/2011-09/2014, lab manager at University of Zurich,
    • Lasani Wijetunge
    • Marilyn Lepleux
    • Mirelle ter Veer
    • Krishna Inavalli
    • Martin Lenz
    • Lotte Razenberg
    • Richard Barrillet
    • Sandrine Pouvreau
    • Thomas Pfeiffer
    • Geraldine Louvet
    • Ezequiel Saraceno
    • Emanuele Murana
    • Cleopatre Djefal
    • Carlotta Pessina
    • Aude Panatier
    • Aleks Ichkova
    • Abdu Kuku